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KMID : 0903520070500040304
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.304 ~ p.307
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Production of Spirulina Extract by Enzymatic Hydrolysis
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In Man-Jin
Gwon Su-Yeon
Chae Hee-Jeong
Kim Dong-Chung
Kim Dong-Ho
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Abstract
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An efficient production method of spirulina extract was developed by enzymatic treatment using cell lytic and proteolytic enzymes. The suitable dosage of Tunicase, a cell lytic enzyme, was found to be 2.0% (w/w). Proteolytic enzymes were screened to obtain high solid recovery and spirulina extraction (SE) index, which indicates nucleic acid-related substances content. Among the seven tested proteases, Esperase was selected and optimal dosage of this enzyme was 2.0% (w/w). The solid recovery and SE index of simultaneous treatment and co-treatment using optimal dosages of Tunicase and Esperase were greatly similar, respectively. However, co-treatment had the effect of shortening total hydrolysis time. The SE index and solid recovery of co-treatment were significantly enhanced by 75% (11.4→20.0) and 45% (45.2%→65.3%), respectively, than those of the non- treated extracts.
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KEYWORD
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enzymatic hydrolysis, solid recovery, spirulina extract
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